Background technology

There are currently no reports on how to achieve simulated high-altitude low-pressure and low oxygen environment cell culture both domestically and internationally. The existing low oxygen culture chamber can only perform low oxygen, high oxygen and other cultures; If low-pressure and low oxygen cell culture is carried out, only a cell culture box can be built into the low-pressure oxygen chamber, but there are many disadvantages: 1. The daily maintenance cost of the low-pressure oxygen chamber is about 4000 yuan or more; 2. There are many inconveniences for researchers to enter and exit the low-pressure oxygen chamber every day, and low-pressure and low oxygen conditions can also cause damage to the researchers' bodies.

Product content

The purpose of this product is to provide a low-pressure and low oxygen cell culture device, aimed at addressing the shortcomings of the aforementioned background technology.

This product is implemented as follows: a low-pressure and low oxygen cell culture device, comprising a low oxygen culture chamber, a negative pressure suction device, a pressure gauge, and an oxygen concentration gauge. The low oxygen culture chamber includes an inlet and an outlet, both of which are equipped with valves; The air inlet is sequentially connected to a pressure gauge, an oxygen concentration gauge, and a negative pressure suction device through a pipeline.

Compared with the shortcomings and deficiencies of existing technologies, this product has the following beneficial effects: it is easy to operate, can simulate cell culture in high-altitude low-pressure and low oxygen environments, is non-invasive to humans, and effectively saves research costs.

Attached image description

Figure 1 is a schematic diagram of the structure of the low-pressure and low oxygen cell culture device of this product.

Embodiment

In order to make the purpose, technical solution, and advantages of this product clearer and more understandable, the following will provide further detailed explanations of this product in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the product and are not intended to limit the product.

As shown in Figure 1, Figure 1 is a schematic diagram of the structure of the low-pressure and low oxygen cell culture device of this product.

A low-pressure and low oxygen cell culture device, comprising a low oxygen culture chamber 1, a negative pressure suction device 2, a pressure gauge 3, and an oxygen concentration gauge 4, wherein the low oxygen culture chamber comprises an inlet 5 and an outlet 6, and valves are installed on both the inlet 5 and the outlet 6; The air inlet 5 is sequentially connected to the pressure gauge 3, oxygen concentration gauge 4, and negative pressure suction device 2 through pipelines.

In the actual application process of this product, the following steps are included:

(1) Open the valves of inlet 5 and outlet 6, and fill the low oxygen culture chamber 1 with premixed gas (such as 2% oxygen, 5% carbon dioxide, 93% nitrogen) for 3-7 minutes.

When both the inlet and outlet are open, pre charge a mixed gas (such as 2% oxygen, 5% carbon dioxide, 93% nitrogen) through the inlet. After pre charging for 3-7 minutes, the culture chamber reaches the pre charged mixed gas concentration state.

(2) Close the valve at inlet 5 of low oxygen culture chamber 1, and connect outlet 6 to negative pressure suction device 2.

(3) Negative pressure suction is carried out through negative pressure suction device 2 according to the preset pressure, and the oxygen concentration can be adjusted by pre filling gas to achieve the ideal low-pressure and low oxygen state.

(4) After reaching the predetermined pressure, clamp the valve of the air outlet 6 and place the low oxygen culture chamber 1 in the cell culture box for cultivation.

This product is easy to operate and can simulate cell culture in high-altitude low-pressure and low oxygen environments. It is non-invasive to humans and effectively saves research costs.