Youpu Jia UHPLC+High Performance Liquid Chromatography System



UHPLC+design concept runs through the entire range of nanoliter liquid phase, conventional liquid phase, and rapid liquid phase

The maximum pressure of basic and standard analytical systems has established a new standard for efficient liquid phase -620 bar

Dual ternary liquid phase system - designed for routine analysis, increases yield and expands the application range of chromatographic analysis technology

Chameleon chromatography software - intelligent, professional, and user-friendly

Viper and nanoViper connector system - can be manually installed and removed, suitable for ultra fast liquid phase range and ensuring zero dead volume.

RSLCnano system

• Provides nanoliter/capillary/microliter flow rate range from 20 nL/min to 50 μ L/min

The maximum pressure resistance of the column can reach 800 bar

Continuous DC transmission

The sample pump can provide a flow rate from 10 μ L/min to 2.5 mL/min



RSLC system

Both binary and quaternary systems can be used for applications involving ultra fast liquid phases and conventional liquid phases

• A wider range of pressure flow rates compatible with ultra fast and ultra-high resolution analytical applications

The maximum withstand voltage of the system is 15000psi

• Can provide efficient dual ternary RSLC system



Standard analytical system

Provide optimal performance and reliability for conventional liquid-phase applications

620bar pressure resistance and 100Hz data acquisition frequency are compatible with ultra fast liquid phase applications

• Flexible configuration according to different applications

The maximum flow rate can reach 10mL/min, meeting the needs of all-round applications

Basic system

• Meet regular application requirements and be more durable

The analysis results are consistent and reliable

• Provides 620bar pressure resistance and up to 100Hz data acquisition frequency, fully compatible with UHPLC applications

The automatic sampler is integrated with the column temperature box, providing sample and chromatographic column temperature control functions

Detector Options

• Provides multiple optical detector options - UV/visible, fluorescence, and differential refractive index detectors

• Innovative Corona universal electric spray detector

High sensitivity Coulomb electrochemical detector

• Supports AB, Bruker, and Thermo Fisher mass spectrometry detectors

Software and spare parts

Chameleon software - intuitive instrument control, advanced data processing

D-Library database provides application search support

Viper and nanoViper hand swivel joint system, truly zero dead volume, simplifies installation and disassembly process

• Column processing and manufacturing - including integral column technology and advanced multi matrix bonding technology

UltiMate ® 3000 RSLCnano system

At present, the focus of RSLCnano system development is to improve sample flux. UltiMate ® The 3000 RSLCnano system adopts a stable and continuous DC transmission method, which can achieve uninterrupted analysis of samples. UltiMate ® The 3000 RSLCnano system has a wide flow rate pressure range, ensuring the highest separation and fastest analysis requirements even for the most complex enzymatic peptide samples at the nanoliter level. It is the only system that can simultaneously meet the requirements of high separation speed, separation capability, and sensitivity. The separation capabilities covering nanoliter, capillary, and microfluidic levels, as well as the powerful dual gradient pump, provide users with excellent flexibility. UltiMate ® The 3000RSLCnano supports higher-level workflows, such as automated offline two-dimensional separation (which utilizes dual pumps, dual switching valves, and automatic sampler for trace component collection).

UltiMate ® 3000 RSLC system

The RSLC system has pushed HPLC to a new realm of high performance and high flexibility. Precise equipment, advanced data processing, and high-quality chemical reagents can easily meet all chromatographic performance challenges. UltiMate ® The 3000 RSLC offers a choice of binary, quaternary, and dual ternary pumps, providing the maximum flexibility in the industry and suitable for a wide range of applications in conventional and ultra fast liquid phases. UltiMate ® The 3000 dual ternary RSLC system can bring us ultra-high sample processing capacity and advanced and convenient sample automation processing functions. Multidimensional LC has excellent selectivity and separation, and the automatic switching between ultra fast liquid phase and conventional liquid phase applications increases the frequency of instrument use. Cooperating with chameleon software can bring unlimited possibilities for your experimental testing!

Standard analytical system

HPLC is the most fundamental tool in chromatographic laboratories. However, the requirements for system functionality may vary greatly depending on the application. UltiMate ® The 3000 standard analytical system is specifically designed to meet current application needs and future challenges. Supports all regular applications and is compatible with ultra fast features, making ultra fast liquid phase no longer out of reach.

Analysis of biological fluid samples using dual ternary online SPE technology

UltiMate ® The 3000 dual ternary system has strong flexibility, can significantly increase sample processing capacity, and brings advanced automation processing technology:

Parallel and series LC bring twice the sample processing capacity

• Can achieve automatic switching between two applications, simple and convenient

Online SPE-LC enables online enrichment and pre separation of samples

• Fully compatible with ultra fast liquid phase performance, reducing operating time and achieving better separation results

Basic automatic system

The basic automatic liquid phase system is the best choice for routine applications: it has high reliability, strong flexibility, and is also fully compatible with ultra fast liquid phases. The ACC-3000 automatic sampler column temperature box integration component is the core part of the system, integrating the sampler with the powerful column temperature box.

Fully meet routine testing needs, more economical

The 620bar pressure resistance and data acquisition rate of up to 100Hz ensure ultra fast liquid-phase compatibility

The reduction of critical connection points reduces the number of vulnerable components

• Multiple detectors can be selected

Automatic sampler and column temperature box unit

UltiMate ® The 3000 automatic sampler provides comprehensive guarantees for the reliability, accuracy, and precision of injection, achieving extremely low cross contamination rates from nanoliters to milliliters. The fraction collector includes both simple sample collection and high-end collection re injection processes (such as automated offline two-dimensional liquid phase applications).

optical detector

UltiMate ® The 3000 optical detector includes UV visible absorption, fluorescence, differential, and evaporative light scattering detectors to meet the detection needs of different analyses. High data acquisition frequency, multi wavelength detection, and 3D ultraviolet spectroscopy can be well compatible with ultra fast liquid phase, better meeting analysis and separation requirements.

Electric spray detector

Corona electric spray detector (CAD) is a universal detector, which can detect any nonvolatile and partially semi volatile substances and is compatible with gradients. C o r o n a Ul t r a provides a data acquisition rate of up to 100Hz and is fully compatible with UHPLC applications. Corona CAD is an ideal choice for conventional HPLC systems.

Coulombic electrochemical detector

Coulochem ® The III electrochemical detector is used for the analysis of oxidizable and reducible substances. Its amperometric, coulombic, pulsed amperometric detection, and cyclic voltammetry all have outstanding detection performance. With the simultaneous introduction of the highly sensitive 6011 dual electrode electrochemical detector and the efficient Coulombic analysis cell, it is fully compatible with ultra fast liquid phase.

mass spectrometry detector

MSQ Plus is currently the smallest and most sensitive quadrupole mass spectrometer detector on the market, supported by chameleon software. In addition, Diane's liquid chromatography can also be connected to other mass spectrometry using DCMSLINK ™ Plugin, available from mass spectrometry software X c a l i b u r (T h e r mo F i s h e r Scientific), Analyst ® AB Sciex and HyStar ™ Use Bruker Daltonics for instrument control.

The pioneering universal joint system

The use of Viper and nanoViper connector systems can avoid various problems caused by the use of conventional connectors, and the perfect connection ensures optimal chromatographic performance.

Zero dead volume, high pressure resistance, manual installation and disassembly. Can be used for nanoliter/capillary, microfluidic, and analytical liquid phase systems

• Compatible with various types and brands of valves and chromatography columns

• Equally compatible with stainless steel flow paths and quartz capillary tubes

All RSLC and dual ternary systems are equipped with an innovative Viper connection system, allowing you to have the best and most convenient liquid-phase system. The NanoViper adapter is a standard accessory for the RSLCnano system and Acclaim PepMap RSLC nanoliter chromatography column.

Powerful chromatography management software

Our chameleon chromatography management software has numerous intelligent functions that can simplify operations and easily meet the needs of operators. A well-designed user-friendly interface can guide users to efficiently establish sequence methods and process data. By using the revolutionary eWorkflow feature, a complete sample sequence and analysis plan can be obtained with just a few selection operations.

The powerful data analysis function of chameleon software ensures the efficient and accurate display, parsing, and reporting process of the entire data. The chameleon 7 software greatly simplifies the process from sample to final detection result, improving work efficiency.

D-Library database

D-Library is an exciting application database that utilizes Web 2.0 technology for easier use. On the user interface, users can search based on different criteria such as analyte, matrix, market, column type, instrument type, or operating time, and quickly find liquid phase and ion application methods. The built-in RSS feed can promptly inform users of the latest application methods. Once a suitable application is found, D-Library will provide users with the corresponding chameleon software method files.