EVOM2It is the latest generation used for specific completion of tissue culture researchTEERThe resistance meter used for measurement, compared to *EVOMCompared to having the characteristics of simpler operation and easier use.EVOM2Not only can it qualitatively measure the health status of single-layer adherent cells, but it can also quantitatively measure the fusion status of single-layer cells.

uniqueEVOM2Circuit Design andSTX2The combination of electrodes enables monitoring of the fusion status of single-layer cells, when combined withWPIWhen combined with a single small chamber designed by the company, it can also be used to achieve more accurate quantitative measurements or lower resistance measurements, such as the blood-brain barrier(BBB)Measurement of endothelial growth resistance.

Working principle

EVOM2The working principle is to generate a10 μAA constant transmembrane current is applied while reversing the polarity of the electrode at a certain frequency, so that there is no charge accumulation on the electrode membraneSTX2The two current electrodes（I1andI2）Make10 μAThe current crosses the cell membrane, and another pair of electrodes（V1andV2）Measurement requirements met10 μAThe voltage required for the current and sends the information to the processor. The processor converts it into ohms according to Ohm's law and displays the number on the reading meter. Because the current is fixed10 μATherefore, it is easy to convert the measurement results. If the membrane resistance is1000 When Ω,10 μAThe voltage at which the current passes should be10mVIn200 When the film resistance is Ω,10 μAThe current will generate2 mVThe voltage; In any case, the energy consumed on the membrane or electrode is very small.

Instrument usage

■ Used for studying drug and nutrient transport in endothelial cells of the blood-brain barrier;

■ used forCaCo-2Pharmacodynamic study of colorectal cancer cells or other mucosal or epithelial cancer cells;

■ Research on drug absorption and nutrient transport in cultured intestinal mucosal cells;