【 Supply Thin Layer Chromatography Spot Quantitative Capillary 】 Complete specifications, accurate quantification!!

1. 2, 3, 4, 5, 10ul;

Thin layer chromatography spot quantification capillary specifications and models: 1, 2, 3, 4, 5, 10ul, 100 per box.

A pipe with a very thin inner diameter is called“capillary”. It usually refers to a thin tube with an inner diameter of 1 millimeter or less, and is called a capillary tube because some tubes have a diameter as thin as hair. For example, mercury thermometers, narrow slits at the tip of pens, gaps between towels and ink absorbing paper fibers, fine gaps in soil structures, and veins in plant roots, stems, and leaves can all be considered capillaries. Capillary electrochromatography is a new type of differential separation analysis technology developed in recent years. It integrates the advantages of capillary electrophoresis and micro column liquid chromatography. By applying a direct current high-voltage electric field at both ends of the micro column chromatography column filled with fine particle liquid chromatography packing, it achieves superior separation ability for complex biological and chemical system samples with trace amounts.

How to improve sampling efficiency?

Sampling is the main source of TLC quantification errors. Experimental results have shown that it is more suitable for smaller volume samples; Micro injectors are more suitable for larger volume samples. This is mainly because microinjectors are greatly affected by small bubbles and solution crawling phenomena. To avoid sampling errors between different quantitative capillaries, it is recommended to use the same quantitative capillary on a thin layer plate. However, it should be noted that when replacing the sample, the capillary tube should be cleaned thoroughly with ultrasonic waves or solvents of different polarities. When preparing samples, the viscosity of the solvent used for sample dissolution should not be too high to facilitate sampling; If the boiling point of the solvent is too low, the injection volume will be variable; if it is too high, it will change the composition of the developing agent; Excessive solubility of the sample can cause hollowing of the sample points; The commonly used solvents are methanol, ethanol, and acetone. The origin of classic TLC sample points is generally 3mm in diameter, with a distance of 1-2cm between points and a distance of 1.5cm from the bottom edge; The origin of HPLC sample points is generally 1mm in diameter, 5mm in distance between points, and 1cm in distance from the bottom edge.