Shimadzu InertSuatao AQ-C18 liquid chromatography column

InertSustain ™ A novel chromatographic column combining C18 high inertness and high retention *

Physical properties of fillers

• Usually, silica gel based chromatography columns have good mechanical stability and can provide high separation efficiency, but they cannot be analyzed under alkaline conditions, and the residual silica gel on their surface is very easy to adsorb alkaline organic compounds.
• InertSustain C18 uses a newly modified silica gel with chemical modifications on its surface, allowing for precise control of the active sites on the surface.
• InertSustain C18 not only inherits the advantages of Inertsil series liquid chromatography columns, such as extremely low column back pressure, high inertness to many analytes, and high column efficiency, but also has good compatibility with many solvents. It can also be analyzed in a wide pH range and has continuous and stable column efficiency between columns of different batches.

*New silicone gel with changes

• It is impossible to completely seal the residual silanol group using traditional chemical modification methods. GL Sciences has developed an innovative silicone gel that provides both high inertness (alkali deactivation) and excellent durability across a wide pH range.

*Step: [Synthesize a new type of silicone gel]

In the synthesis process of ES new silica gel, the distribution of active sites and silanol groups can be strictly controlled, so InertSustain C18 can provide high inertness to almost all substances even under acidic and alkaline mobile phase conditions.

Step 2: [Bonding ODS chemical groups]

The adoption of ES new silicone gel and our company's chemical bonding technology enable InertSustain C18 to have strong retention ability for hydrophobic compounds, while also being able to withstand 100% aqueous mobile phase.

Step 3: [End capped]

GL Sciences' end capped technology enables InertSustain C18 to provide higher column efficiency and superior peak shapes even when analyzing compounds with strong adsorption properties.

advantage

Adopting a new type of silicone matrix

The introduction of ES new silicone enables the development of more effective methods for InertSustain C18 over a wide pH range, opening up the possibility of selective changes.

Better peak shape and better separation

Due to the precise control of the surface active sites and silanol group distribution of ES new silica gel, InertSustain C18 can provide highly stable analytical spectra for qualitative and quantitative analysis of all compounds.

Wide pH compatibility and better tolerance

InertSustain C18 has better durability in acidic and alkaline mobile phases and can withstand pH ranges of 1-10. Some samples require high pH conditions for dissolution operations to maintain analytical stability, and InertSutain C18 is the * choice for analyzing these samples.

Strict quality management ensures better repeatability

The entire production process is operated under strict quality management in the Japanese factory to ensure reliable and stable column efficiency for different batches of chromatography columns.

Operating back pressure

InertSustain C18 can provide lower operating back pressure, even with high viscosity solvents, resulting in extremely low operating pressure.

The quality and column efficiency of InertSustain

• When the chromatographic column contains residual active adsorption sites, it can cause peak tailing or sample adsorption problems. This phenomenon does not occur on InertSustain C18 because the silanol group is tail sealed and has a neutral silica gel surface.
• In order to ensure the reproducibility of the product, strict chromatographic testing was carried out on each chromatographic column, examining inertness, durability, theoretical plate number, and compound retention time reproducibility.

Basic compound

Huanglian Su and Dexmedetomidine are two highly alkaline compounds that can cause severe peak tailing when silanol groups remain on the surface of the chromatographic column packing. As shown in the figure on the right, InertSustain can provide very stable chromatograms for qualitative and quantitative analysis.

Acid Compounds

When the surface of the chromatographic column packing appears alkaline, it can cause tailing of two acidic compounds, bright blue FCF and salicylic acid. InertSustain can provide excellent peak shapes due to its neutral silica gel surface.

Longer column lifespan and wider pH compatibility

As shown in the experimental results below, due to the introduction of the new ES silica gel, InertSustain C18 can maintain high column efficiency and sharp peak shape for 300 hours, which cannot be achieved by other brands' wide pH range chromatography columns.

Stability, reproducibility, column efficiency, and quality

Strict quality control of filler properties and detection of chromatographic filler inertness and selectivity spectra ensure that each InertSustain C18 chromatographic column has outstanding reproducibility and a long column life.

Stable reproducibility

Strict quality control of filler properties and detection of chromatographic filler inertness and selectivity spectra ensure that each InertSustain C18 chromatographic column has outstanding reproducibility and a long column life.

Shimadzu InertSuatao AQ-C18 liquid chromatography column

analytical column

Traditional protection column: replaceable column core protection column series

Universal protection column WondaGuard C18

preparative column

Prepare column protection column

Compatible with Waters1/16 connectors.
※ Other specifications of chromatography can be customized according to customer requirements.

Shimadzu InertSuatao AQ-C18 liquid chromatography column Application examples