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Shimadzu GL InertSep HLB Solid Phase Extraction Column
The Shimadzu GL InertSep HLB solid-phase extraction column has developed a novel hydrophilic and lipophilic co balanced polymer reverse phase packing
Product details
Shimadzu GL InertSep HLB Solid Phase Extraction ColumnProduct description
New hydrophilic and lipophilic co balanced polymer reverse phase fillers developed
● High specific surface area, wide pH tolerance range, and strong universality
● Higher recovery rate, stronger reproducibility
●
product structure
| particle size | ：30 μm |
| surface area | ：720 m2/g |
| Micro pore volume | ：1.3 mL/g |
| Micro pore diameter | 70 |
| PH range | ： 1-14 |
Shimadzu GL InertSep HLB Solid Phase Extraction ColumnProduct performance comparison data
Conclusion: From the comparison chart, it can be seen that the elution curve and recovery rate data of the new HLB column are comparable to the performance of the commercially available W company column.
Shimadzu GL InertSep HLB Solid Phase Extraction ColumnOrder Information
| Product Name | Specifications | packaging unit | Item Number |
|---|---|---|---|
| InertSep HLB | 30 mg/1 mL | 100 pieces | 5010-81971 |
| 60 mg/3 mL | 100 pieces | 5010-81973 | |
| 150 mg/6 mL | 30 pieces | 5010-81975 | |
| 200 mg/6 mL | 30 pieces | 5010-81976 | |
| 500 mg/12 mL | 30 pieces | 5010-81977 |
application
Application example of InertSep new product - detection of tetracycline veterinary drugs in grass carp
Application examples of InertSep's new products
Sample pre-treatment method:
Weigh 2.0g of the sample, add 8mL of buffer solution and 2mL of 20g/L lead acetate solution, vortex for 1 minute, centrifuge at 8000rpm for 10 minutes, and set aside the supernatant. Repeat the above steps twice for the residue, merge and make up to 25mL. After removing the fat from 10mL of n-hexane, weigh 12.5mL of the clear solution for purification.
Buffer solution *: Weigh 10.9g of disodium hydrogen phosphate (Na2HPO4 · 12H2O), 12.9g of citric acid (C6H8O7 · H2O), and 37.2g of disodium ethylenediaminetetraacetic acid, dissolve in water, dilute to 1000mL, and adjust the pH to 4.0 with NaOH.
Analysis conditions:
Instrument: Shimadzu LC-30A combined with LCMS-8050 system
Chromatographic column: Shim pack XR-ODS III, 75 × 2.0 mm, 1.6 μ m (P/N: 228-59922-92)
Flow rate: 0.3mL/min
Injection volume: 1 μ L
Column temperature: 35 ℃
Mobile phase: A: 0.2% formic acid water B: acetonitrile
The gradient elution procedure is as follows:
| Time (Min) | 0 | 3 | 4 | 10 |
|---|---|---|---|---|
| A（%） | 85 | 65 | 85 | 85 |
| B（%） | 15 | 35 | 15 | 15 |
Mass spectrometry conditions:
Ionization mode: ESI
Scanning method: Positive ion scanning
Detection method: Multi reaction monitoring
Ion spray voltage: 4.0KV
Atomizing gas: nitrogen 3L/min
Dry gas: nitrogen 10L/min Colliding gas: argon DL
Temperature: 250 ℃
Heating module temperature: 400 ℃
MRM chromatogram of standard sample
Comparison data of experimental results and recovery rates
| experimental results | InertSep HLB 60 mg/3 mL | W Company HLB 60 mg/3 mL | ||||||
|---|---|---|---|---|---|---|---|---|
| Recovery rate (%) | Oxytetracycline | tetracycline | Chlortetracycline | Doxycycline | Oxytetracycline | tetracycline | Chlortetracycline | Doxycycline |
| 84.65 | 84.30 | 79.73 | 84.41 | 89.98 | 73.02 | 74.27 | 83.05 | |
| 87.09 | 80.61 | 77.49 | 86.33 | 87.35 | 82.67 | 72.94 | 82.90 | |
| 87.05 | 80.41 | 72.75 | 88.11 | 97.32 | 73.52 | 70.47 | 80.19 | |
| Average recovery rate (%) | 86.26 | 81.77 | 76.66 | 85.92 | 91.55 | 76.40 | 72.56 | 82.05 |
| RSD(%) | 1.62 | 2.68 | 4.65 | 2.15 | 5.64 | 7.11 | 2.66 | 1.97 |
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