ND2000 Ultra Micro Spectrophotometer
NanoDrop 2000Ultra trace ultraviolet\\Visible spectrophotometercorrectNanoDropThe new product utilizes the surface tension characteristics of liquids, and the sample volume only requires0.5~2ulOn the detection platform, the fixed optical path is pulled out through the contact of the upper and lower arms to achieve the advantages of fast, trace, high concentration, no quartz tube, no capillary tube and other consumable detection absorption values. This product design is protected and widely popular worldwide, and its accuracy and convenience allow scientists to conduct various research more efficiently.
NanoDrop 2000Use high-energy xenon lamp(pulsed xenon flash lamp)Available190~840nmFull spectrum detection without the need for warm-up, use immediately after startup. Paired with high sensitivityCCD arrayDetector, capable of detecting absorption values up to300Abs(dsDNAconcentration2~15000ng/ul)Most purified nucleic acids can be detected almost without dilution.
The homogeneity of the sample to be tested isNanoDrop 2000High requirements for nucleic acid and protein samples are generally met before testingvortex mixerIt's better to shake evenly, if notvortex mixerIt should also bepipetteSuck and mix several times. If you're worriedgenomic DNAIf it may break due to the aforementioned action, then change it to55?CHeat for about one minute to ensure a uniform state of the sample before detection2ulRepresentative.
By selecting different detection modes during testing, fast results can be obtained:
● Nucleic Acid –Absorption spectra230nm, 260nm, 280nmabsorbance(Convert to10mmOptical path absorption value)、260/280 ratio、260/230 ratioAnd the concentration of nucleic acid.
● UV-Vis – 190~840nmAbsorption values and spectra of all wavelengths between(use1mmPresentation of optical path absorption values).
● A280Protein quantification method– 280nmabsorbance(Convert to10mmOptical path absorption value)、260/280 ratioAnd protein concentration. Only applicable to purified proteins, must have a known mass extinction coefficient(mass extinction coefficient)Only then can it be calculated.
● BCA、BradfordreachLowry –Provide different wavelength absorption values based on different colorants, automatically draw standard curves and calculate themR squareThe protein concentration to be tested.
* The protein detection mode is currently availableBCA、BradfordreachLowryThree common quantitative colorants, as colorants gradually deepen over time, please read the reagent instructions carefully before use and prepare standard samples of different concentrations to complete the detection quickly and obtain a good standard curve. Each standard curve can have up to seven standard samples, and each standard sample can have up to five replicates.
* When measuring proteins, the sample volume needs to be used2ulAfter testing each sample, it is necessary toKimwipesLow dust wiping paper(Number:34155 )rub15~20To avoid residual colorants and proteins.
ND2000 Ultra Micro SpectrophotometerConcentration range:
Sample type |
Low concentration |
high concentration (Please dilute the sample when exceeded) |
reproducibility(Five or more repetitions) |
nucleic acid |
2 ng/ul |
15000 ng/ul (dsDNA) 12000 ng/ul (RNA) |
The concentration range is2-100 ng/ulWhen,SDdo± 2 ng/ul The concentration range is greater than100 ng/ulWhen,CVdo± 2% |
pureBSA |
0.10 mg/ml |
100 mg/ml |
The concentration range is0.05-10 mg/mlWhen,SDdo± 0.10 mg/ml Concentration greater than10 mg/mlWhen,CVdo± 2% |
Protein, toBCAQuantitative Method |
0.2 mg/ml |
8.0 mg/ml |
CV:± 2% (All within the detectable concentration range) |
Protein, tomodified LowryQuantitative Method |
0.2 mg/ml |
4.0 mg/ml |
CV:± 2% (All within the detectable concentration range) |
Protein, toBradfordQuantitative Method |
100 ug/ml |
8000 ug/ml |
The concentration range is100-500 ug/mlWhen,SDdo± 25 ug/ml Concentration greater than500 ug/mlWhen,CVdo± 5% |
Protein, toMini BradfordQuantitative Method |
15 ug/ml |
100 ug/ml |
The concentration range is15-50 ug/mlWhen,SDdo± 4 ug/ml Concentration greater than50 ug/mlWhen,CVdo± 5% |
Product Description:
NanoDrop ND-2000CdoNanoDrop ND-2000The upgraded version,ND-2000ChaveND-2000In addition to all its functions, it also has the following outstanding features:
1Shorter detection time, only requires3second;
2There are two options: colorimetric cup or detection hole, suitable for detecting various types of samples, including the detection of volatile substances;
3The detection range is wider, and the detection lower limit can be as low as0.4 ng/μl (dsDNA);
4Built in colorimetric cup for real-time dynamic curve research, orOD600Measurement of cell concentration.
Technical parameters:
During the measurement of inspection holes:
1Wavelength Range: 190-840nm;
2Wavelength accuracy: 1nm;
3Resolution: < 1.8 nm (FWHM at Hg 253.7 nm);
4Other: 1mmoptical path length(Automatically adjustable to0.05mm);
5Detection limit:2ng/μl(dsDNA);
6Detection upper limit:15000ng/μl(dsDNA);
7Accuracy of absorbance:0.002 absorbance (1mmoptical path);
8Accuracy of absorbance:2%(at 0.76 at 257 nm);
9Range of absorbance:0.02-300 (equivalent to10mmoptical path);
10Nucleic acid testing cycle:< 5s;
11Volume:14cm×20cm.
When measuring with a colorimetric cup:
1Beam height:8.5 mm;
2Accurate temperature control,37 ± 0.5 °C;
3Mixing speed: 150 – 850 rpm;
4There are four optical paths to choose from, namely1,2,5,10mm;
5Range of absorbance:0.002 – 1.5;
6Detection limit:0.4 ng/μl (dsDNA);
7Detection upper limit:750 ng/μl (dsDNA);
8Testing time:< 3 s;
9Weight:2.1 kg.
Welcome new and old customers to purchase