RocheLightCycler480 Fluorescence Quantitative Roche PCR InstrumentIt allows you to achieve real-time online high-throughput rapid fluorescence quantitative PCR cycle, which can simultaneously detect 96 or 384 well plate samples. The results can be quantitatively and genotypically analyzed through real-time fluorescence collection and software analysis during the PCR cycle. Complex optical detection systems can perform multiplex PCR detection and are suitable for multiple detection modes. Over 10 years of rich experience in the development of real-time quantitative PCR systems and reagents have converged into the pinnacle of today's industry.

OneLightCycler480 Fluorescence Quantitative Roche PCR InstrumentMain features:
*High sensitivity: capable of detecting single copy genes
*Wide dynamic range: can simultaneously detect 1-1010 copies of DNA
*High repeatability: CV%<0.15%
*High resolution: easily distinguish the concentration difference between 1000 and 2000 copies
*High speed: Complete 40 PCR cycles in 40 minutes (384 well plate)
*Technological innovation: Adopting Therma BaseTM thermal cycling technology and advanced optical detection system, completely eliminating edge effects and maintaining stable and excellent detection results.
*Omnipotent: Various analysis modes, including absolute quantification, relative quantification, genotyping (dissolution curve method and endpoint method), high-resolution dissolution curve analysis, and applicable to almost all detection modes (dye detection, hydrolysis probe (TaqMan probe), hybridization probe, simple probe, etc.)
*Open platform: can use the vast majority of fluorescent dyes on the market as well as 8-tube, 96 well plate, and 384 well plate provided by third parties
*Flexible: interchangeable 96 hole and 384 hole heating modules, users can replace them themselves without the need for engineers to be present
*Automation platform: equipped with LIMS system and automatic injection robotic arm, it can achieve remote operation and fully automated operation.

Widely used in absolute quantification, relative quantification, endpoint genotyping, dissolution curve genotyping, DNA methylation research, microRNA research, HRM gene scanning and other studies.

IIInstrument parameters:
*Sample wells: 96 or 384
*Size Width: 57.4cm x Length: 58.8cm x Height: 49.7cm
*Weight: 55.6 kilograms
*Power: 200-240Vac (50/60Hz, 1500VA)
*Reaction volume: 5ul -20ul (384 wells), 10ul -100ul (96 wells)
*Uniformity of spatial temperature: ± 0.1 ℃
*Maximum heating rate: 4.8 ℃/S
*Maximum cooling rate: 2.5 ℃/S
*Excitation light source: standard xenon lamp
*Detection device: Cold CCD
*Filter excitation wavelength (nm): 450783523558615
*Emission wavelength (nm): 500533, 568610640670
*Computer: Pentium computer, pre installed with Windows XP
*Software function: Absolute quantification Tm calling、 Relative quantification with efficiency correction, genotyping, product identification, etc
*Automation performance (requires additional Light Cycler) ® 480 System LIMS/Bar Code Software Module):
-Equipped with LIMS interface
-Multi hole plate scanning and recognition can be performed through barcode
-Compatible with automatic loading equipment for porous plates
*Data Management: Compliant with 21 CFR-11

III Related products:
Roche has developed a universal kit for probes, SYBR Green I, and genotyping on the LightCycler 480 system.
– LightCycler 480 SYBR Green I Master
– LightCycler 480 Probes Master
– LightCycler 480 Genotyping Master

4 Universal probe library UPL and specialized prefabricated probe combination library

Related product item number
LightCycler ® 480 fully automatic fluorescence quantitative PCR system (including 96 well module) 05 015 278 001
LightCycler ® 480 fully automatic fluorescence quantitative PCR system (including 384 well module) 05 015 243 001
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LightCycler ® 480 384 well plate, white 04 729 749 001
LightCycler ® 480 96 well plate, transparent 05 102 413 001
LightCycler ® 480 384 well plate, transparent 05 102 430 001
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LightCycler ® 480 Genotyping Master 04 707 524 001
LightCycler ® 480 Control Kit 04 701 924 001