Leica microscope accessoriesLight source

The illumination used for microscopic observation has a significant impact on the final image quality, and the choice of illumination that can present the effect depends on the type of sample, the relevant characteristics of the sample, and the application field and purpose of microscopic observation.

　　Leica microscopeLED lighting technology has been fully adopted, and compared with commonly used halogen lamps, LED microscope lighting technology can provide several advantages for microscopic imaging, including:

1. Longer lifespan (25000 to 50000 hours);

2. Lower power consumption;

3. Natural color temperature;

4. Cold light source → less heat release (useful for temperature sensitive samples);

5. Practical compact design;

6. It can maintain a constant color temperature even at different brightness levels.

　　When choosing the correct lighting type, several important factors should be considered for high-quality microscopic observation and imaging of the sample:

1. What type of sample should be observed;

2. What characteristics of the sample should be analyzed;

3. What are the shortcomings of using the current lighting type;

4. Is it necessary to manipulate the sample during microscopic observation, such as using cutting tools, tweezers, soldering iron, or other tools.

Backlight illumination (BLI) provides transmissive illumination for transparent samples.

Results of Lighting with Leica LED5000 and LED3000

The following are image examples of various samples. These images were recorded using a Leica M165 stereomicroscope equipped with a Leica DFC495 digital camera and LED3000 or LED5000 series illumination system.

Sample: Microelectronics

Other recommendations

When choosing a lighting system, in addition to the high-quality optical components used in Leica Microsystems microscopes, it is also important to determine the sample characteristics to be analyzed and the field of view required for observation (i.e. field of view).

In addition, it is necessary to consider the advantages of computer coding and appropriate microscope optical performance, such as flat field, apochromatic, and achromatic objective lenses.

Please remember to also take into account other lighting factors:

·In order to achieve the required optical performance, some lighting systems may be incompatible with each other, for example, the Leica LED5000 RL lighting system cannot use a 2.0x objective lens.

·Alternative lighting systems must be considered, such as using Leica LED point light sources (SLI) with gooseneck systems instead of Leica LED ring lights (RL) when using stereomicroscopes with objective turntables or microscope configurations with small working distances.