The wide field high-resolution hard X-ray white light microscopy coupled optical imaging system is mainly used in life science research. During the research process, X-rays are used to penetrate biological tissue samples, and fluorescence images are excited by scintillation crystals. The micro coupled optical imaging system amplifies the weak fluorescence images and transmits them to the large-area photoelectric imaging system to form real-time biological tissue activity images for scientific research.

The difference between the white light microscopy coupled optical imaging system used in applications and conventional microscopy systems lies in its wide microscopy imaging field and large numerical aperture. At the same time, in order to avoid damage to the lens caused by high-dose X-rays penetrating biological tissues, it is necessary to consider high-intensity radiation resistance requirements in the design.

25X design parameters

System magnification: 25X

Maximum numerical aperture of the object: 0.7

Band range: 0.52 μ m to 0.58 μ m

Object working distance: 2.16mm

Maximum field of view of the image: ± 45mm

System length: 577mm

Maximum diameter: 70mm

25x microscope dimensions 521cm X 349cm X 511cm

Light path diagram

At a spatial frequency of 1000lp/mm (within 90% of the full field of view) at 0.5 microns, the spatial modulation transfer function (design index average) MTF is ≥ 0.4.

Dot chart

Field curvature and distortion

Maximum distortion of system design: ≤ 0.5% (within 90% of the full field of view)

Physical picture

MTF parameter testing

25X discrimination rate board imaging grayscale calculation

From the above data, it can be calculated