IntelliQube-Global supplier

8th Floor, Building 2, East China Normal University Science and Technology Park, 1038 Jinshajiang Road, Shanghai, China

Fully automated PCR system establishment, amplification, and analysis system!
IntelliQube is a fully automated and high-throughput instrument that seamlessly integrates processes such as liquid handling, thermal cycling, detection, and data analysis. IntelliQube supports quantitative real-time PCR, endpoint PCR, and isothermal chemical methods, allowing you to fully enjoy the innovative Array Tape^®The convenient experience of inline automation brought by consumables.




integration
IntelliQube integrates various instruments used in the PCR process, simplifying your workflow, achieving unmanned operation, simplified loading and running, saving laboratory space, and allowing researchers to focus more on the research itself rather than instrument management.

efficiency
IntelliQube and Array Tape consumables can provide scalable high-throughput solutions for your laboratory. Compared to traditional 384 well qPCR instruments, the new 768 well specification can double the PCR throughput. IntelliQube and Hydrocycler for endpoint based genotype testing^TMThe combination can process up to 50 microplates or 19000 reactions per day!

economic
The reaction system is small (1.6uL), and you can save about 90% of the cost.

flexibility
IntelliQube is compatible with various chemical and fluorescent probes. The integration of liquid processing has improved operational flexibility. Compared to chip or microfluidic methods, it reduces waste. A series of qPCR and endpoint PCR applications are compatible with this system, including but not limited to: l Gene expression analysis l Genotyping and Copy Number Analysis (CNVs) l Microbial detection and quantitative analysis l miRNA analysis l Conjugability and GMO testing

high-performance
IntelliQube has industry-leading liquid handling, thermal cycling, and optical systems that can provide the accurate PCR results you expect. Its flexibility, efficiency, and integration fully meet the needs of your laboratory.

liquid handling system
IntelliQube's top-notch multifunctional pipetting system facilitates rapid liquid handling of 384 well or 768 well Array Tapes. The operation of transferring samples to Array Tape is done through interchangeable CyBi^®The Felix 96 or 384 channel pipette head is used, while the 4-channel pipette injector can transfer reagents into the reaction system. The error of the IntelliQube liquid processing system is less than 5%, ensuring the reproducibility and data quality of the experiment.


Pipette flushing
The IntelliQube pipette flushing system can effectively reduce the consumption of pipette tips, lower the cost of corresponding consumables, and reduce the risk of cross contamination. This system utilizes the fluid stirring of the pipette head to achieve complete cleaning of the inside and outside of the suction head, while its touch off function can counteract surface tension and remove residual liquid.Combining cleaning additives such as bleach with each cleaning can ensure the integrity of the test and reduce the risk of cross contamination.
Liquid Handling Standards

thermal cycle
IntelliQube supports inline amplification through a Peltier thermal cycling block specifically designed for Array Tape. Due to the unique block design and surface geometry, there is no need to replace the block, and it can support 384 hole array tape and 768 hole array tape. This system has excellent heating rate, uniformity and accuracy of reaction temperature, and can be widely compatible with various amplification methods, including rapid PCR and isothermal chemical methods.

Hydrocycler^TM
Hydrocycler combines flux, high efficiency, and stable design characteristics to inline amplify endpoint PCR reactants on array coils, making it another high-throughput alternative. The system has 3 accurately temperature controlled water baths, which can be optimized to 50 arrays for rapid thermal cycling per operation. The endpoint fluorescence signal can be read out by IntelliQube after the PCR reaction is complete, which is 5 times higher in endpoint PCR flux compared to inline thermal cycling.

detection
IntelliQube has advanced optical systems that enable inline fluorescence detection on Arrays Tape. The filtered LED has been optimized for various commonly used fluorescent groups with excitation ranges of 480-620 nm. The high-resolution CCD camera supports detecting dye fluorescence intensity within the emission range of 510-705nm in 5 detection channels.

Testing standards

Software and Data Analysis
Intellics is an innovative software product that provides central data management, instrument monitoring, operational optimization, protocol generation, and simplified data analysis for IntelliQube. Intellics is preloaded on each instrument, and users can access it through a unified web-based interface that allows for simple multi-user access. Intellics has two distinctive applications: Array Builder ™ Allow users to quickly generate liquid handling and thermal cycling protocols; IntelliScore can be used for post PCR data analysis and supports various types of PCR experiments, including absolute quantification (standard curve), relative quantification (relative standard curve and Δ Δ Cq), genotype analysis, etc. The touch screen interface is convenient for instrument control and also supports real-time data monitoring.
Instrument control and software specifications
Figure 1: Amplification curves of 5000 and 10000 copies of gDNA at 2x resolution
Figure 2: Significant Cq value variation curve obtained for the entire array analysis of 10000 copies of gDNA.
picture3：10Doubly diluted plasmidDNAWide dynamic range of amplification curve
Other performance specifications

Place of Origin:LGC UK