Wanshen HiCC-Z fully automatic colony counting, inhibition zone measurement, and sulbactam sensitive β - lactam enzyme analyzer

1、 Usage: Used for automatic microbial and colony counting analysis, inhibition zone measurement, antibiotic susceptibility potency analysis, as well as β - lactam enzyme detection and analysis, lactobacillus potency analysis, etc

2、 Main performance indicators:

2.1 ★ Imaging and Light Source: Dual light source LED color backlight, 6-dish scanning imaging with dark field of view, maximum optical resolution of 9600 × 4800dpi, capable of achieving 29.6 million pixel imaging effect, maximum color depth of 48 bits, adjustable imaging brightness, automatic focusing, and controllable color temperature. Simultaneously measure 6 90mm petri dishes, suitable for target analysis of 50-180mm circular plates and 280 × 180mm oversized rectangular plates for cultivation.

2.2 Characteristics of colony counting analysis

1) ★ Automatic recognition and statistics: SmartdownTM colony intelligent recognition technology,Having the characteristic of automatic enhancement of colony targets,Automatically identify large scale clustered and chain like adhered elongated bacteria (microscopic morphology of large adhered Escherichia coli, bacterial spores) and various bacterial colonies on petri dishes (including Staphylococcus aureus count (GB4789.10-2016), Escherichia coli community determination (GB4789.3-2016), etc.). It can automatically form a batch processing wizard and achieve one click automatic counting.Equipped with a large depth of field 8-megapixel autofocus camera for more effective color selectionAutomatic classification and counting of bacterial colonies.

2) The automatic counting accuracy is ≥ 96.5%, with a maximum monitoring correction of 3.5%, achieving 100% accuracy. Analysis and statistics speed: 150-800 colonies/s, with a total imaging and counting time of ≤ 5 seconds per dish for a large volume of 6 dishes

3) ★ Colony adhesion segmentation: Automatically segment long, circular and other colony targets that adhere to each other in patches, and users can choose to segment or not segment. It is also possible to manually segment and merge colony target images

4) ★ Color and Shape Recognition: With fast level set extraction technology, it can recognize and count specific colors and shapes according to 20 categories, and can be corrected by category conversion. 6 target areas can be selected simultaneously for automatic analysis, and the analysis target areas can be freely increased, decreased, or edited

5) Colony morphology analysis: Automatically obtain colony area, equivalent diameter, major axis, minor axis, aspect ratio, roundness, perimeter, shape factor, etc. You can filter and query based on morphological indicators.

6) Mouse click correction, seamless removal of grids and text, automatic removal of impurities, effective support for complex microbial statistics, in compliance with the parameter automatic conversion characteristics of GB4789.2-2016.

2.3 Measurement of inhibition zone (analysis of antibiotic susceptibility potency):

1) ★ One click fully automatic determination of all antibacterial zones in 6 90mm diameter petri dishes, which can automatically determine locally adhered antibacterial zones. In the presence of local text interference, the antibacterial zone area, diameter, and other results can be automatically obtained. Analysis takes 1-4 seconds and can save or view various types of analysis data in a unified manner

2) The petri dish can be placed in any direction. It has the functions of automatic selection of antibacterial zone samples, accurate visualization comparison, and editing. The reading resolution of the antibacterial zone measurement system is ≤ 0.001mm, the highest optical measurement resolution is ≤ 0.0053mm, and the repeated measurement error is ≤± 0.01mm or ≤± 0.1%; The measurement error of potency is ≤± 0.1%; Repeated measurement error of potency ≤ ± 0.1%; Measurement difference between stations ≤ 0.2%

3) ★ Complies with the Chinese Pharmacopoeia, USP United States Pharmacopeia, and EP European Pharmacopoeia, and can complete the latest National Pharmacopoeia (2020 edition) first, second, and third dose and combined calculations, as well as antibiotic potency analysis for all strains. Combination optimization analysis characteristics with potency.

4) Built in with the latest 25th edition (2015) of the American CLSI "Antimicrobial Susceptibility Test Execution Standard", providing a data input and modification platform for easy updates, and providing a quick tool for paper based drug susceptibility analysis.

5) According to the food additive Streptococcus lactiae QB 2394-2007 standard, more than 24 inhibition zones of a large rectangular plate can be automatically measured at once and the potency can be automatically analyzed.

2.4 ★ Sulbactam sensitive β - lactase test

1) According to the national Ministry of Health's "Method for the Detection of Sulbactam Sensitive β - Lactamases in Milk and Dairy Products - Cup Plate Method", measure the content of sulbactam sensitive β - lactamases (Jinyulanase) in milk and dairy products. The diameter of the inhibition zone generated by tubes (A), (B), and (D) in the pure water control can be used to automatically determine whether the analysis results of the six petri dishes are valid. A positive or negative result output report can be automatically generated based on the diameter of the inhibition zone generated by tubes (A) and (B) in the sample.

2) It has an automatic warning function for invalid reports, which can automatically remove impurities or defects based on differences in size, making the report more comprehensive and reliable. It can generate reports that comply with GLP/GMP requirements, adhere to the integrity of raw data and records, and provide comprehensive data support for laboratory certification. Historical data can be accessed freely, facilitating digital file management in the laboratory.

2.5 Data export and query, etc.:The analysis of images and data results can be saved and automatically generated into Excel or PDF document reports. Various types of analysis data and image evidence can be saved or viewed uniformly, and a total report can be formed. Automatic size calibration and manual calibration. It has functions such as automatic selection of antibacterial zone samples, visual comparison, interactive precise measurement, and editing. The latest Windows operating interface is easy to use, and the software can be upgraded online independently.

2.6 ★ Data traceability, permission protection, traceable operation records, in compliance with GLP and GMP requirements：

Adopting a multi system architecture with separate functions and permissions to ensure the security, integrity, authenticity, and traceability of data information.

1) System administrator:Responsible for creating and managing accounts for all regular administrators and operators, and setting permissions for regular administrators and operators. There is only one system administrator who has the highest authority in the system.

2) Ordinary administrator:Responsible for reviewing and archiving all test data, as well as managing other system data. systemMultiple regular administrators can be assignedThe permissions are set and allocated by the system administrator.

3) Operator:Responsible for sample production, testing, correction, generating electronic reports, submitting for review, etc. systemMultiple operators can be assignedThe permissions are set and allocated by the system administrator.

4) All accounts can change their login password, set their own electronic signature, and output it to the report.

5) The system automatically records the operation records of each account, and the system administrator can trace, view, and output the operation records.

2.7 Configuration

1) 1 ScanMaker i800 Plus dual light source color scanning imaging device andOne set of 8-megapixel autofocus camera with a large depth of field

2) Wanshen fully automatic colony counting analysis, inhibition zone measurement, antibiotic susceptibility potency analysis, intelligent β - lactase detection and analysis software and lock 1 set

3) One branded all-in-one computer (Core Dual Core CPU/4GB RAM/1GB RAM/500GB hard drive/23 "color display/wireless network card, Windows XP or Windows 7 system)

4) Wanshen HiCC series automatic antibacterial zone measuring instrument1 dedicated standard board (with 1 authoritative calibration certificate attached)

Note: The amount marked with ★ in this technical proposal must be responded to, otherwise it is a significant deviation