1、 Usage: Used for automatic microbial and colony counting analysis, inhibition zone measurement, antibiotic susceptibility potency analysis, as well as lactostreptomycin potency analysis, microscopic cell counting analysis, etc

2、 ★ Imaging device

1) The Zhongjing ScanMaker i800 Plus has dual LED light sources for color scanning imaging, with adjustable brightness and automatic focusing. It can achieve uniform backlight and dark field imaging effects for 29.6 million pixel images, with a maximum color depth of 48 bits.

 2) Adaptation to culture dishes: rectangular plates within 50-180mm and A4 size, simultaneously measuring 6 90mm plates (pouring, coating, membrane filtration, spiral plates, 3M paper sheets)

3) Imported Olympus CX31 three eye biological microscope for bright field observation (professional grade 10 megapixel camera imaging)

3、 Colony count analysis

1) ★ Automatic recognition and statistics: SmartdownTM colony intelligent recognition technology,Having the characteristic of automatic enhancement of colony targets,Automatically identify large scale clustered and chain like adhered elongated bacteria (microscopic morphology of large adhered Escherichia coli, bacterial spores) and various bacterial colonies on petri dishes (including Staphylococcus aureus count (GB4789.10-2016), Escherichia coli community determination (GB4789.3-2016), etc.).It can automatically form a batch processing wizard and achieve one click automatic counting.Equipped with a large depth of field 8-megapixel autofocus camera for more effective automatic classification and counting of bacterial colonies.

2) The automatic counting accuracy is ≥ 96.5%, with a maximum monitoring correction of 3.5%, achieving 100% accuracy. Analysis and statistics speed: 150-800 colonies/s, with a total imaging and counting time of ≤ 5 seconds per dish for a large volume of 6 dishes

3) ★ Colony adhesion segmentation: Automatically segment long, circular and other colony targets that adhere to each other in patches, and users can choose to segment or not segment. It is also possible to manually segment and merge colony target images

4) ★ Color and shape recognition:Equipped with fast level set extraction technologyIt can identify and count the number of colonies of specific colors and shapes according to 20 categories, and can be corrected by category conversion. 6 target areas can be selected simultaneously for automatic analysis, and the analysis target areas can be freely increased, decreased, or edited

5) Colony morphology analysis: Automatically obtain colony area, equivalent diameter, major axis, minor axis, aspect ratio, roundness, perimeter, shape factor, etc. You can filter and query based on morphological indicators.

6) Mouse click correction,Untrace removal of grids and textAutomatically remove impurities, effectively support complex microbial statistics, and comply with the parameter automatic conversion characteristics of GB4789.2-2016.

4、 Antibacterial zone measurement

1) One click fully automatic determination of all antibacterial zones in 6 90mm diameter petri dishes, capable of fully detecting locally adhered antibacterial zones, and automatically obtaining results such as antibacterial zone area and diameter in the presence of local text interference. Measurement and analysis take 1-4 seconds, and can save or view various types of analysis data uniformly

2) The petri dish can be placed in any direction. It has the functions of automatic selection of antibacterial zone samples, accurate visualization comparison, and editing. The reading resolution of the antibacterial zone measurement system is ≤ 0.001mm, the highest optical measurement resolution is ≤ 0.0053mm, and the repeated measurement error is ≤± 0.01mm or ≤± 0.1%; The measurement error of potency is ≤± 0.1%; Repeated measurement error of potency ≤ ± 0.1%; Measurement difference between stations ≤ 0.2%

3) ★ Complies with the Chinese Pharmacopoeia, USP United States Pharmacopeia, and EP European Pharmacopoeia, and can complete the latest National Pharmacopoeia (2020 edition) first, second, and third dose and combined calculations, as well as antibiotic potency analysis for all strains. Combination optimization analysis characteristics with potency.

4) According to the QB 2394-2007 standard for food additive Streptococcus lactiae, more than 24 inhibition zones can be automatically measured simultaneously and the potency can be automatically analyzed

5) Built in with the latest 25th edition (2015) of the American CLSI "Antimicrobial Susceptibility Test Execution Standard", providing a data input and modification platform for easy updates, and providing a quick tool for paper based drug susceptibility analysis

5、 Microscopic cell counting analysis:

1) Image acquisition management, real-time preview saturation warning

2) ★ Automatic collage and ultra large depth of field expansion feature: Automatically collage to obtain the entire image from multiple fields of view. Automatically generate multi focus 3D high-definition images from images captured on multiple focal planes

3) Image observation and comparison, selection of interest target areas, and enhancement of effects characteristics

4) ★ Automatic correction of light field non-uniformity (automatic background correction), color adjustment, image color separation and filtering, etc

5) Image measurement: measuring line segments, multiple line segments, spline curves, arcs, circles, ellipses, rectangles, polygons, irregular shapes, lengths, areas, perimeters, angles, etc., with caliper tools. Online calibration of measurement units, magnetic lasso, vectorization of all shapes, editable, and arbitrary annotation on images (boundaries, numbers, symbols, fillers, etc.)

6) ★ Automatically segment into long or circular targets with adhesive patches, automatically classify, count, and measure (obtain morphological parameters such as area, perimeter, diameter, main axis, and secondary axis)

7) ★ Automatic learning classification feature by color and shape, interactive conversion of classification categories, and classification enhancement function based on expert guidance for secondary self-learning

8) Analyze image sorting output (based on parameters such as area, size, aspect ratio, etc.) and size distribution histogram output, perform statistics by region or mouse click, edit results separately, query or filter characteristics

6、 Data export, etc.: The analysis of images and data results can be saved and automatically generate Excel or PDF document reports. The software has an online upgrade feature.

7、 Measurement function: Fully automatic size calibration, manual calibration for microscopic analysis. Accurate measurement of length, angle, radian, area, arc, and any curve can be achieved by dragging with the mouse.

8★ Data traceability, permission protection, traceable operation records, in compliance with GLP and GMP requirements：

Adopting a multi system architecture with separate functions and permissions to ensure the security, integrity, authenticity, and traceability of data information.

1) System administrator:Responsible for creating and managing accounts for all regular administrators and operators, and setting permissions for regular administrators and operators. There is only one system administrator who has the highest authority in the system.

2) Ordinary administrator:Responsible for reviewing and archiving all test data, as well as managing other system data. The system can assign multiple regular administrators, whose permissions are set and assigned by the system administrator.

3) Operator:Responsible for sample production, testing, correction, generating electronic reports, submitting for review, etc. The system can assign multiple operators, whose permissions are set and assigned by the system administrator.

4) All accounts can change their login password, set their own electronic signature, and output it to the report.

5) The system automatically records the operation records of each account, and the system administrator can trace, view, and output the operation records.

1) 1 ScanMaker i800 Plus dual light source color scanning imaging deviceAnd the large depth of field with autofocus800Ten thousand pixel shooting device1cover

2) Olympus CX31 Trinocular Biological Microscope (10 megapixel camera imaging) 1 unit

3) Fully automatic colony counting analysis, antibacterial zone measurement analysis, cell counting analysis software and lock 1 set

4) One branded all-in-one computer (Core Dual Core/4GB RAM/1GB RAM/500GB hard drive/23 "color display/wireless network card, Windows operating system)

Note: The amount marked with ★ in this technical proposal must be responded to, otherwise it is a significant deviation