Phytosterols are a general term for a large class of chemical substances that exist in plants. Their structure is similar to cholesterol and they are almost present in all plant-based foods. Common ones includeβ-Sitosterol, brassinosteroids, and stigmasterol, followed by the saturated form of plant sterols, namelyβ-Tanistanol and rapeseed oil stanol, etc. A large amount of epidemiological data and laboratory studies have shown that a higher intake of plant sterols is associated with a lower incidence of many chronic diseases in the population, such as reducing the risk of hypercholesterolemia, coronary heart disease, cancer, and other diseases. Therefore, establishing analysis methods for plant sterols, determining the content of plant sterols in food, and estimating the intake of residents are of great significance. They can provide basic data for the rational utilization of plant-based foods in China and also provide a basis for scientifically guiding residents' diets.

Tengzhou Luchuang Analysis Co., LtdGB/T25223-2010/ISO12228-1999Standard for Determination of Sterol Composition and Total Sterol Content in Animal and Plant Oils and Fats by Gas Chromatography (International Organization for Standardization)ISOThis method is also used as the standard method for analyzing plant sterolsEstablished a gas chromatography analysis method for plant sterols(GClaw)And utilize itGCAnalyzed the law7General categories of agreements150The content and distribution of plant sterols in common plant-based foods were analyzed, and the dietary intake of plant sterols in Chinese residents was estimated using data on the content of plant sterols in common foods. stem or root of plantsschemeFirstly, we explored the pretreatment methods for common plant-based foods, including acid hydrolysis, saponification, extraction, and derivatization conditions, while optimizing the production of plant sterolsGCAnalyze the conditions; Selected vegetable oils, grains, beans, vegetables, fruits, potatoes, nuts, etc7General categories of agreements150A food sample, utilizingGCAnalyzed the lawβ-Sitosterol, brassinosteroid, stigmasterolβ-Calculate the content of sitosterol and brassinosterol, as well as the total plant sterol content and the relative content of each sterol.Luchuang Chromatography Laboratorystudyindicate:Utilize optimized pre-processing methods andGCAnalysis conditions show that the separation efficiency of various sterols is good, and there are few impurity peaks; Vegetable oil contains brassinosteroids, stigmasterolβ-The recovery rates of sitosterol are as follows:100.4%The93.3%The103.3%. Acid hydrolysis of brassinosteroids and stigmasterol in the sample is requiredβ-The recovery rates of sitosterol are as follows:94.6%The92.7%The

1、 Main features of the instrument:

1、GC-9860gas chromatographAdopting linear optimization of the two door exterior design and a fully microcomputer automatic control system,Adjustable Chinese high-definition LCD display screen, displaying rich and intuitive content; Chinese keyboard style operation, setting parameters and operation are very convenient.
2 New DoubleCPUDesign and import highly integrated electronic circuit technology with original packaging. The application of highly integrated chips enables the instrument to have excellent reliability and anti-interference ability,The precision of temperature control is high.
3Design of multi gas path and large volume column chamber,*Multiple columns can be installed simultaneously with three chromatography columnsFID、TCDTwo types of detectors, And it can be easily expandedECD , FPD , NPDThree types of detectors, as well as external control equipment such as an expanded headspace sampler, gas sampler, and conversion furnace, have been used in one machine for multiple purposes.
4Can be equipped with two packed column vaporization chambers simultaneously(PIP)Split the flow with an independent capillary tube/Non diversion dedicated system（SPL）The design of the vaporization chamber draws on the miniaturization design of similar foreign products, with fast heating speed and no injection dead corners,Has excellent capillary diversion capability/Non diversion sampler（SPL）The tail blowing system can easily install small-diameter capillary columns, large-diameter capillary columns, and various filling columns.
5Equipped with a five order linear program heating function,Satisfied with multi-component sample analysis. The column chamber is equipped with a variable frequency intelligent rear door automatic cooling system, The cooling rate is very fast280℃drop to50℃It only needs5minute)Realized near room temperature operation in the sense of * *.
6Equipped with six independent temperature control systems.Suitable for installing multiple temperature control requirements such as conversion furnaces;Column chamber, gasification chamber, hydrogen flame detection chamber, thermal conductivity detection chamber, auxiliary1Assist2All adopt modular design,Installation and replacement are more convenient.
7Equipped with power failure protection function and automatic memory setting parameters. It has anti power mutation interference function and stopwatch timing function, and is designed with multiple self correction functions.

8Having0℃”andOvertemperature protection function, with a temperature of“0Automatically stop heating and sound an alarm when the temperature exceeds the set upper limit; And there are prompts for the cause of the malfunction and alarm prompts. Among similar products, * * gas cut-off protection and prompt function can provide * * limited protectionTCDThe tungsten wire and chromatographic column are not damaged.
9Wide range fault self diagnosis function,36Plant self diagnostic information, display the cause of the fault and its handling methods at any time.
2、 Instrument technical parameters:
Column temperature: Temperature control range: Room temperature+10℃～399℃
Temperature control accuracy: superior to±0.1℃
Temperature gradient: The effective area of the column is not greater than2%
The deviation between the set temperature and the displayed temperature should not exceed1℃
The deviation between the set temperature and the actual temperature shall not exceed2%
gas change Room: Temperature control accuracy:±0.1℃(Room temperature)+15℃～200℃）； greater than200℃do±0.2℃
inspect measure Room: Temperature control accuracy:±0.1℃(Room temperature)+15℃～200℃）； greater than200℃do±0.2℃

3、 Main configuration of the instrument:

Configuration Table of Gas Chromatography for Plant Sterol Analysis