It is generally used in various microscopes to enable people to observe various fluorescence phenomena more conveniently. The microscopes commonly used for optical filters include fluorescence microscopy, laser scanning confocal fluorescence microscopy (LSCM), confocal microscopy, and total internal reflection fluorescence microscopy (TIRFM); Mainly used in the fields of life sciences and biomedical sciences, its main function is to separate and select the characteristic spectral bands of excitation light and emission fluorescence of substances in biomedical fluorescence detection and analysis systems.