FluorCamPortable chlorophyll fluorescence imaging device

The FluorCam portable chlorophyll fluorescence imaging instrument is designed for dynamic analysis of fluorescence parameters imaging of leaves and small plants in the field and laboratory. In all applications, the system can image the fluorescence transient processes induced by photochemical and saturation light, and the time and intensity of photochemical light irradiation can be determined by user-defined programs. The software package contains various commonly used experimental programs and simple, practical, and powerful programming languages, allowing users to design their own flash sequences and measurement processes.

The FluorCam portable chlorophyll fluorescence imaging system is a lightweight and portable system that is particularly suitable for field experiments and can also be used for laboratory work. The system can be powered in the field through a dedicated battery pack, and the stable and lightweight tripod makes field measurements easier and more feasible.

Features:

·Strong portability, suitable for both laboratory and field use

· You can edit the measurement experiment program by yourself（protocol）

· Equipped with automatic repeated measurement function

· It can perform both continuous photochemical imaging measurements andPAMImaging measurement

· Equipped with dark adaptation leaf clips, it can measure samples without damage

·Optional high-resolution lens with snapshot mode and video mode（binningMode)

· Optional handheld chlorophyll rapid fluorescence kinetics measurement module

Typical sample

·Algae such as kelp, horsetail algae, seaweed, blue-green algae communities, etc

·Lichens, mosses, crusts, etc

·Whole small plants, such as Arabidopsis, etc

·Plant canopy, leaves or fruits, such as strawberries, cucumbers, tomatoes, lemons, melons, etc

· Other photosynthetic organisms

Technical Specifications

• Measurement parameters: Fo, Fo’,Fs, Fm, Fm’, Fp, FtDn, FtLn, Fv, Fv'/ Fm'，Fv/Fm ,Fv',Ft,ΦPSII, NPQ_Dn,NPQ_Ln, Qp_Dn, Qp_Ln, qN, qP，QY,QY_Ln, Rfd, ETR Waiting for over 50 chlorophyll fluorescence parameters, each parameter can display a 2D fluorescence color image

·Equipped with a complete automatic measurement program (protocol), the automatic measurement program can be freely edited

a) Fv/Fm: Measurement parameters include Fo, Fm, Fv, QY, etc

b) Kautsky induced effect: Fluorescence parameters such as Fo, Fp, Fv, Ft_Lss, QY, Rfd, etc

c) Fluorescence quenching analysis: More than 50 parameters including Fo, Fm, Fp, Fs, Fv, QY, Φ II, NPQ, Qp, Rfd, qL, etc

d) Light response curve LC: Fluorescence parameters such as Fo, Fm, QY, QYLn, ETR, etc

• High sensitivity TOMI-1 CCD sensor:

a) Image resolution: 720 × 560 pixels

b) Time resolution: 50 frames per second

c) A/D conversion resolution: 12 bits (4096 grayscale levels)

d) Pixel size: 8.6 µ m × 8.3 µ m

e) Communication mode: Gigabit Ethernet

High resolution TOMI-2 CCD sensor (optional)

a) Line by line scanning CCD

b) Maximum image resolution: 1360 × 1024 pixels

c) Time resolution: up to 20 frames per second at the highest image resolution

d) A/D conversion resolution: 16 bits (65536 grayscale levels)

e) Pixel size: 6.45 µ m × 6.45 µ m

f) Operation mode: 1) Dynamic video mode, used for measuring chlorophyll fluorescence parameters; 2) Snapshot mode, used for measuring fluorescent proteins such as GFP and fluorescent dyes

g) Communication mode: Gigabit Ethernet

• Imaging area: 35mm × 46mm

• Light source board: 4 ultra bright LED light source boards, each light source board consists of a 5 × 5 LED array, with a size of 4 × 4 cm

• Measurement light: 620nm red light, duration adjustable from 10 µ s to 100 µ s

• Saturated light: Standard white light, optional blue light (455nm) or red light (620nm)
  White light: up to 3900 µ mol (photons)/m ². s

Blue light: up to 4900 µ mol (photons)/m ². s
Red light: up to 3800 µ mol (photons)/m ². s

• Photochemical light: Standard white light, optional blue light (455nm) or red light (620nm)
  White light: 0-1000 µ mol (photons)/m ². s

Blue light: 0-1400 µ mol (photons)/m ². s
Red light: 0-800 µ mol (photons)/m ². s

• Far red light: 735nm, used for measuring Fo ', 4 high-energy LEDs

• OJIP – test (optional): can analyze the rapid fluorescence dynamic photochemical and thermal phases of plants

• FluorCam chlorophyll fluorescence imaging analysis software features: Live (live testing), Protocols (customized experimental program selection), Pre processing (imaging preprocessing), Result (imaging analysis results) and other menu functions

• Customized experimental program protocols for customers: can set time (such as measurement light duration, photochemical light duration, measurement time, etc.), light intensity (such as photochemical light intensity of different qualities, saturation light flash intensity, modulated measurement light, etc.), with dedicated experimental program language and scripts. Users can also freely create new experimental programs using the wizard program template in the Protocol menu

• Automatic measurement and analysis function: An experimental program (Protocol) can be set to automatically perform unmanned cyclic imaging measurements. The number of repetitions and interval time can be customized by the customer, and the imaging measurement data will be automatically stored in the computer according to the time and date (with a timestamp)

• Snapshot mode: Through snapshot imaging mode, the light intensity, shutter time, and sensitivity can be freely adjusted to obtain clear and prominent steady-state and instantaneous fluorescence images of plant samples

• Imaging preprocessing: The program software can automatically identify multiple plant samples or regions, or manually select regions of interest (ROI). The shape of manual selection can be square, circular, any polygon, or sector. The software can automatically measure and analyze the fluorescence kinetics curves and corresponding parameters of each sample and selected area, with no limit on the number of samples or areas (>1000)

• Data analysis mode: It has "signal calculation and re averaging" mode (arithmetic mean) and "signal average and re calculation" mode. In high signal-to-noise ratio situations, the "signal calculation and re averaging" mode is selected, and in low signal-to-noise ratio situations, the "signal average and re calculation" mode is selected to filter out errors caused by noise.

• Output results: High time resolution fluorescence dynamic image, fluorescence dynamic change video, fluorescence parameter Excel file, histogram, imaging images with different parameters, fluorescence parameter list for different ROIs, etc

• Light giving system: static or dynamic (sinus style)

• CCD detection range: 400-1000nm

• Spectral response: The quantum efficiency is highest at 540nm (70%), and decreases by 50% at 400nm and 650nm

• Read out noise: below 12eRMS, typical 10e

• Full trap capacity: greater than 70000 e (unbound)

• Bios: Firmware upgradable

• Communication method: Gigabit Ethernet

• Host weight: 1.8 kg

• Host size: 21.5 cm × 13.5 cm × 13.5 cm

• Leaf clamp: used to clamp measuring blades and perform dark adaptation

• Bracket system: 1) Indoor bracket, adjustable measurement height and angle, used for laboratory measurements; 2) Triangle bracket (optional), designed with waterproof and rust proof materials to meet measurement stability, adjustable height angle, maximum measurement height of 1.5m, used for field measurement

• Power supply method: 1) 90-240 V AC power, equipped with a dedicated surge resistant and stabilized power supply; 2) Specialized field battery pack (optional), capable of supporting uninterrupted measurement for over 10 hours on a single charge

• Maximum power: 200 W

Place of OriginEurope