Beckman flow cytometer sheath fluid， Beckman Coulter CytoFLEX sheath fluid, flow cytometer sheath fluid.
Order number: B51503
Description: CytoFLEX sheath Fluid (no discount) 10L/barrel
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Beckman flow cytometer sheath fluidIt is a balanced electrolyte solution without a fluorescent background, mainly composed of sodium chloride, potassium chloride, disodium ethylenediaminetetraacetic acid, and antibacterial agents. It is used as a sheath fluid for analyzing the physicochemical and biological characteristics of biological particles such as cells using a Coulter flow cytometer. Most blood analyzers use the sheath flow principle to improve detection accuracy, and the main reagent used is sheath fluid. The forms of sheath flow include single sheath flow and multiple sheath flow, both of which aim to make cells pass through the detection section in a straight line.

mechanism

In the sheath flow cell (usually divided into front and back sheath flow cells or inner and outer sheath flow cells), positive pressure is used to inject the sheath fluid. The sheath fluid forms a fixed direction vortex in the sheath flow cell. At this time, the sample is injected in the form of positive pressure. All specimens can only flow through the sheath flow cell in the flow direction without exception, which forms the sheath flow direction. Resistance counting, absorbance counting, or light scattering counting in this direction can fully ensure the correct counting of each cell and ensure sufficient passage quantity. It can also ensure that the counted sample will not flow back. Due to sheath flow protection, the sample will pass through the counting tube or hole at the center of the sheath flow, so there will be no deviation interference signal, which can ensure the accuracy of each cell. Free.

The influence of sheath fluid on detection results

Due to the need for the sheath fluid to wrap around the tested sample and undergo laser irradiation or electrical impedance counting by the testing department, the cleanliness, absorbance, pH value, and osmotic pressure of the sheath fluid must meet the design requirements of the instrument, otherwise they will have an impact on the detection results of the instrument.