Agilent liquid chromatography column ProSEC 300SFeatures:
ProSEC 300S Biomolecular Separation and Analysis Chromatography Column
Agilent ProSEC 300S is a silicone based filler specifically designed for protein analysis using volume exclusion chromatography. The surface modification used by ProSEC fillers to remove acidic silanol groups and reduce interactions has minimal non volume exclusion effects on various proteins. The new ProSEC 300S packing material has a labeled pore size of 300 Å and is suitable for the analysis of various spherical proteins. Its 5 µ m particle size gives it high column efficiency and enables higher separation. When using buffer salt eluent, this packing material is an ideal choice for all protein analyses.
Agilent liquid chromatography column ProSEC 300S feature
- Silicone fillers with stable mechanical properties will not be lost during use
- A single chromatographic column that expands the linear separation range
- Column specifications suitable for multi detector systems
performance metrics
| Bonding phase | aperture | particle size | Protein molecular weight range | pH range | Max pressure | flow velocity |
| ProSEC 300S | 300O | 5 µm | 1500-800000 | 2-7.5 | 250 bar, 3700 psi | <1.5 mL/min (7.5 mm inner diameter) |
| <0.5 mL/min (4.6 mm inner diameter) |
