Name: Agilent Bio IEX liquid chromatography column
Brand: Suzhou Chuangpu Scientific Instrument Co., Ltd
Availability: InStock

503B, Building 2, No. 1099 Baodai West Road, Hengtang, Suzhou High tech Zone (Huijin Science and Technology Innovation Center)

Agilent Bio IEX liquid chromatography column Features:Agilent Bio ion Exchange HPLC ColumnFilled with polymer based non porous ion exchange fillers. The biological ion exchange chromatography column is designed specifically for achieving high separation, recovery, and efficient separation of peptides, oligonucleotides, and proteins. The Bio IEX series chromatography columns offer strong cation exchange (SCX), weak cation exchange (WCX), strong anion exchange (SAX), and weak anion exchange (WAX) fillers. All biological ion exchange stationary phases are available with non porous fillers with particle sizes of 1.7, 3, 5, and 10 μ m.

Agilent Bio IEX liquid chromatography columnfeature

Highly cross-linked non porous polystyrene divinylbenzene (PS/DVB) hard filler surface combined with hydrophilic polymer layer, avoiding non-specific bonding
Uniformly and densely packed ion exchange functional groups are chemically bonded to the hydrophilic layer (with multiple ion exchange groups connected to each site), increasing the capacity of the chromatographic column
Fillers, coatings, and bonds can withstand high pressure, which helps achieve higher separation and faster separation.
Multiple ion exchange groups are fixedly attached to a binding site to increase column capacity.

Agilent Bio IEX liquid chromatography columnperformance metrics

Chromatographic column stationary phase	SCX (Strong Cation Exchange) - SO₃H WCX (weak cation exchange) - COOH SAX (Strong Anion Exchange) - N (CH)₃)₃ WAX-N (Weak Anion Exchange) - N (C)₂H₅)₂
load	Non porous, poly (styrene divinylbenzene) (PS/DVB) with hydrophobic coating and uniformly bonded cation exchange layer
particle size	1.7、3、5 和 10 μm
pore structure	Non porous
PH stability	2-12
Upper limit of operating temperature	80 ºC
Upper limit of operating pressure for column hardware	600 bar (8700 psi) suitable for stainless steel column hardware 400 bar (5800 psi) suitable for PEEK column hardware
Upper limit of packing operation pressure	10 μ m packing at 275 bar (4000 psi) 5 μ m packing 413 bar (6000 psi) 3 μ m packing 551 bar (8000 psi) 1.7 μ m packing 689 bar (10000 psi)
Mobile phase compatibility	Compatible with aqueous buffer, acetonitrile/acetone/methanol, and water mixtures. Common buffer solutions: phosphate, tris, MES, and acetate
Working flow rate	The flow rate for a 4.6 mm inner diameter column or a 2.1 mm inner diameter column is generally 0.1-1.0 mL/min: start with a low flow rate and set the lower value of the hardware and/or packing pressure upper limit as the default value.
Dynamic combination capacity		NP3	NP5	NP10
	SCX	53 mg/mL	38 mg/mL	20 mg/mL
	WCX	19 mg/mL	15 mg/mL	10 mg/mL
	SAX	35 mg/mL	28 mg/mL	17 mg/mL
	WAX	26 mg/mL	18 mg/mL	12 mg/mL