Advanced Electrophoresis Solutions Ltd (AES)A high-tech company in the fields of life sciences and protein chemistry located in Ontario, Canada, has launched a new generation capillary electrophoresis system-Capillary electrophoresis isoelectric aggregation-Full column imaging detection technology（cIEF-WCID）,Thoroughly overturned the definition of traditional electrophoresis technology in protein analysis, bycIEF-WCIDTechnology, separation of complex proteins andpIThe measurement of points has become unusually easy. Becoming a powerful tool for the development and quality control of protein and antibody drugs, basic research in proteomics, and exploration of biological interaction mechanisms. It is also a new technological means for functional protein detection, breeding, dairy products, meat, and other aspects of food safety.

Technical features:
1)Can obtain ultra-high protein contentpIResolution of points(△pI<0.01)
2)Ultra high-throughput protein and antibody drug analysis(Analysis time<10 min)
3) CEInfiniteuseCMOS(Complementary Metal-Oxide-SemicondutorTranslatorComplementary Metal Oxide Semiconductors)As an imaging detection technology, it replaces traditional methodsCCDImaging technology has advantages such as high-speed data acquisition, wide linear range, green energy saving, ultra-high detection sensitivity, and instrument stability, which are important foundations of industrial standards
4) CEInfiniteAn automatic sampling system with ultra-high stability(Alcotte719)And powerful operation and data processing software with automatic data matchingpIFunctions such as automatic software calculation of values
5)Currently, all over the worldpIMarker(pI Marker): 30speciespI Marker(pI point from 2.8 to11);perfectpHcarrier(carrier ampholytes)Unique to four proprietary technologiesAeslytes pH 3-10TheAeslytes pH 2.5-5TheAeslytes pH 5-8AndAeslytes pH 8-10.5, and can customize high-resolution according to customer needspHRange
6)Complete and diversified coated capillary tubesCartridgeGreatly improving the sensitivity and resolution of protein separation
7)The installation and maintenance of the instrument are simple and fast
8)Complete protein and antibody analysis solutions and pre-sales and after-sales teams
9)Small size and light weight(14kg .)Suitable for on-site portable analysis;Green and low energy consumption(only1Electricity consumption per watt)

Technical principles

CEInfiniteThe display shows the protein sample in the entire capillary separation column (length:50 mmInner diameter:100 - 200 μm）The concentration distribution at different separation time points internally. When the capillary tube is filled with a biphasic electrolyte and sample, high voltage is applied to start focusing,CMOSThe imaging sensor continuously performs ultraviolet absorption imaging on the entire capillary tube (imaging time interval)10 - 60 s）UntilcIEFAnalysis completed, the entire process is approximately5-10 min.

Ultra high work efficiency
CEInfiniteEliminating traditioncIEFThe movement process and gel ofIEFThe dyeing process and analysis time can be shortened to15 min /Sample, resolution up to0.01 pH.CEInfiniteThe ultra fast analysis speed and full process visualization make the development of protein drug analysis methods extremely simple. The development time of the method has been shortened from the traditional few months to a few hours, and the development of ordinary monoclonal antibody methods only requires1 It is a powerful tool for separating and quantifying proteins, peptides, bacteria, viruses, vaccines, and charge inhomogeneity due to its small size and ease of standardized operation, providing consistent data analysis results.

Main features
•High scanning rateCMOSImage sensor, fast imaging speed, high resolution
•Long lifespanLEDUV light source, lifespan2 Years and above, saving maintenance costs
•Fully automatic high-throughput sample operation, high quantitative accuracy, and good repeatability
•Graphic software interface, easy to operate
•Compliant withFDA 21 CFR PART 11The requirements

Wide range of application directions
CEInfiniteThe system strictly followsFDA 21 CFR PART 11Regulatory requirements. Its excellent repeatability, ultra-high resolution, and fast analysis speed can provide the best protein analysis method for biopharmaceutical enterprise users, helping companies shorten the drug development cycle and accelerate drug market speed. It can be widely used in drug research and development, clone screening, production process monitoring, drug formulation research and analysis, drug stability research, quality control analysis, etc.

Comprehensive biological consumables assistant enables your laboratory to carry out work better and faster

Amphoteric electrolyte carrier
Bipolar electrolyte carriers are the most important part of capillary electrophoresis for separating and detecting proteins. Excellent electrolyte carrier for both sexespHLinear gradient and low protein interaction achieve excellent protein separation and haveGood reproducibility and low UV absorption ensure minimal baseline noise.

Capillary separation column
A wide variety of capillary separation columns to meet your analytical needs.
•Multiple types of separation columns（FC/DB/PA）Separation of proteins with different properties
•Provide100 μmInner diameter and200 μmSeparation columns with two specifications of inner diameter
• 200 μmThe sensitivity ratio of the inner diameter separation column100 μmThe inner diameter of the separation column has been increased100%
•The separation column with a large inner diameter has the characteristics of low injection resistance, less clogging, and faster injection speed

pIMarker
Rich small molecule markers help you achieve accurate determination and quality control of the isoelectric point of protein drugs
• 32PlantingpIMarker
• pIFan Wei Kuan（2.8-11）
•Small molecule design, structurally stable