ABI StepOnePlus real-time fluorescence quantitative PCR instrument from the United StatesApplication:

1Instrument application:SNP(Single nucleotide polymorphism) genotyping, gene expression analysisMicroRNAExpression analysis, chromosome translocation analysis, gene detection, viral load analysis;

2Software application: Absolute quantification based on standard curve, relative standard curve, comparison basedCtRelative quantification of values, melting curve analysis, existence/Not present (positive)/Negative analysis (including positive internal control), genotyping based on or not based on real-time amplification.

Fluorescence detection:

StepOneAll sample reaction wells in the amplifier use a high-power blue colorLEDLighting. shouldLEDsustainable use10In the year, the need to replace light sources has been reduced. Fluorescence emission is detected through a filter on an electric diode. The emission filter can be optimized to work togetherFAM^TM/SYBR^®Green I、VIC／JOE^TMandROX^TMUse of fluorescent dyes.

Software:

American Applied Biosystems CorporationStepOnereal timePCRThe software equipped with the amplifier isWindowsXP^®Run on the operating system and provide functions such as instrument control, data acquisition, and data analysis. The software adopts a user-friendly and intuitive interface, including the following functions:

*Experimental design guide to help you design and set up experiments.

*Transfer plan and formula for rapid experimental setup.

*Advanced settings, suitable for advanced users who require flexibility for more complex applications such as multiple reactions.

*Quick start settings allow you to initiate experiments immediately and react to board information later.

*Real time monitoring of the amplification growth curve allows you to view the experimental progress in real-time.

*Remote real-time monitoring, which can monitor the experimental process from a remote computer.

*Email notification to remind you when the experiment has started or ended.

*The automatic baseline and white motion threshold functions simplify data analysis.

*The automatic single nucleotide polymorphism genotyping recognition function has intuitive chart output and value allocation characteristics.

*Tool tip function makes it easy to identify sample reaction wells when viewing amplification curves or single nucleotide polymorphism genotyping maps.

*Troubleshooting markers help you diagnose and solve problems that arise during experiments.

*Multi graph view, capable of evaluating data from four perspectives simultaneously.

*Convenient cutting and pasting functions.

*Easy export asPowerPoint^®、Excel^®Format or export directly asjpegFile.

ABI StepOnePlus real-time fluorescence quantitative PCR instrument from the United StatesInstrument specifications:

VeriFlex^TMHeating block: six independent temperature control zones

Thermal cycle system: Peltier effect system（Peltier-based System）

Heating block specifications:96Hole heating block

Supported capacity:10-30µl

Supporting consumables:96Hole（0.1ml）Reaction plate and optical cover film96Hole（0.1ml）Reaction plate and optical flat cover8Connecting pipes（0.1ml）Optical flat cover, single tube（0.1ml）Optical flat cover

Sample heating and cooling rate: fast mode ±2.2℃/sStandard mode ±1.6℃/s

Heating block heating and cooling rate:4.6℃/s

Temperature range:4℃-100℃

Temperature accuracy: set value/Displaying temperature±0.25℃（35~95℃) (After the clock is started)3minStart measuring)

Temperature uniformity:±0.50℃ (after the clock starts)30s）Temperature range35℃~95℃

Chain breaking curve resolution: as low as0.1℃

Optical system:LEDExcitation light source, emission filter, photodiode

Corrected dye during installation:FAM^TM、SYBR^®Green、VIC^®、JOE^TM、NED、TAMRA、ROX^TMdye

Passive reference dye:ROX^TMdye

Data collection: Collect all reaction hole data from all filters (regardless of the reaction plate settings). The reaction plate settings can be modified after the experiment is completed

fixed quantityPCRAnalysis time: Fast not more than40minute; standard not more than2hour

touch screen:LCD/6.5″ Full VGA（640×480）/32Kcolour

Size: Width 24.6cm（9.7″）Depth 51.2cm（20.2″）Height 42.7cm（16.8″）Weight 23.6kg（52lb.）

TaqMan^®Gene expression,SNP(Single nucleotide polymorphism) genotyping andMicroRNAanalysis

Technical parameters:

1Performance indicators:

Dynamic range:9A linear dynamic range of orders of magnitude

Sensitivity: Can be reported on a single geneTaqMananalytical30µlDetection in reaction volume10Copy copiesRNasePtemplate

Precision: UseTaqMan^®RNase PInstrument validation reaction plate,StepOneAmplification instrument can distinguish5000~10000individualRNase PTemplate Copy (Confidence Level)99.7%）

Run time: fast, easy to useTaqMan^®RNase PVerify the test reaction board,40cyclePCRInsufficient reaction time40min; Standards,40cyclePCRInsufficient reaction time2h

Installation specifications: Before shipment, each unitStepOneThe amplifiers have been professionally calibrated to ensure optical and thermal accuracy. During the installation period of less than four hours, throughRNase PThe instrument validation test reaction board will verify various performance specifications to ensure high accuracy of data.

2No needPCAnd it can be operated online.

StepOneThe amplifier can be installed in five different configurations:

① Local Collaboration Configuration (Computer Control)

② Independently run configuration (without the need for a computer)

③Networking configuration

④Local Collaboration Configuration (LAN Computer Control)

⑤Amplification instrument networking combined with wood ground collaborative computer configuration (computer control)

Connecting to Ethernet enables remote monitoring, downloading, and uploading of test files and data.

3Computer specifications

althoughStepOneThe amplifier can also perform operations without a computer (the experiment can be started from the instrument touch screen through the quick start function), butStepOneThe amplifier may be equipped with a laptop or stand up computerUSBMemory stick. We also provide amplifiers without computers.

The computer provided by the user must meet the following low requirements to support this amplifier:

·WdindowsXPOperating system and installedServicePack2

·Intel processor, clock speed1GHzor higher

·Small memory capacity512MB

·Small hard drive capacity20GB

·CD-ROMoptical disk drive

·Ethernet interface adapter (network card)（10BASE-T）

·Low resolution up to1024×1280The monitor

4Convenient online query:

For the convenience of real-time orderingPCRThe mother liquor and plastic utensils consumables for amplification can be obtained through the amplification softwareDesign Wizard(Design Wizard) Access the list of experimental materials and directly link to the product warehouse of Applied Biosystems in the United States (optional).

Service and Warranty:

buyStepOne^TMreal timePCRThe amplifier includes—Free one-year warranty service (including parts warranty and repair hours).