Product Introduction

THUNDER Imager can provide you with solutions suitable for advanced 3D cell culture experiments, whether you want to study stem cells, globular cell clusters, or organoids.

Benefit from the following advantages:

High throughput can achieve better statistical and workflow efficiency
The instrument is easy to use and has high imaging performance
Optimize physiological conditions to obtain meaningful results
The THUNDER Imager adopts Leica's innovative Computational Clearing technology, which can effectively remove non focal plane blur information in real time, enabling high-quality imaging of 3D samples on camera based fluorescence microscopes. The high sensitivity of the system ensures low phototoxicity and low quenching, comprehensively optimizing conditions to achieve higher image quality.
High throughput can achieve better statistical and workflow efficiency

Automated 3D cell culture experiments for efficient research of next-generation disease models. THUNDER can assist you in high-speed imaging of large volume samples such as lung organs. In addition, automation can also minimize user operation steps in tedious experiments.
Optimize physiological conditions - minimum exposure

For 3D cell culture, following true physiology is the primary condition for obtaining meaningful results. Usually, you want to study cells when they are close to their natural state by optimizing experimental conditions, that is, to achieve the lowest possible light intensity and shortest exposure time.

The THENER Imager 3D Live Cell and THENER Imager 3D Cell Culture meet these needs with a high-end LED source that has a small bandwidth optimized for excitation light. A sensitive high-end sCMOS camera with a quantum efficiency of up to 82%, capable of transmitting important image information even in low light conditions and short exposure times.

In order to further reduce sample exposure, the illumination is limited to the actual recording time. The camera shutter is synchronized with the high-speed (switching time of 20 µ s) Lumencor LED light source to minimize light quenching.
Maintain consistent velocity with live flow for imaging - cellular processes

The metabolic process in vivo is extremely fast, especially for single-cell dimensions. Nowadays, most live cell imaging experiments are conducted on high-speed imaging systems.

The THENER Imager 3D Live Cell and THENER Imager 3D Cell Culture can complete full frame capture in one go, allowing you to experience the powerful capabilities of highly sensitive fluorescence systems based on sCMOS cameras.

Combined with their high sensitivity, THENER Imager 3D Live Cell and THENER Imager 3D Cell Culture can achieve data capture speeds of up to 90 frames per second, helping you observe rapid cell activity. Even deep into thick 3D cell clusters, it can quickly capture clear image data. Thanks to the fast switchable external color filter dial (<27 ms), you can always master the fast imaging process even during experiments with multiple emission wavelengths.
Maintain an appropriate environment while observing live cell cultures

THENER Imager 3D Live Cell and THENER Imager 3D Cell Culture have all the functions needed to keep your cells in a near natural state. The incubator can ensure ideal physiological conditions for live cell cultures, such as system stability, humidity, temperature, and carbon dioxide levels (pH).

With a miniature automatic water dispenser, even if you are conducting long-term experiments, you can use a water immersion objective to perform multi position workflows. The immersion objective lens has higher light collection performance, resulting in higher contrast and resolution of the captured cell images.
Easy control of intermittent uptake in multi position experiments: tracking cellular changes

THENER Imager 3D Live Cell and THENER Imager 3D Cell Culture provide both speed and reliability for your 3D live cell culture experiments at multiple locations. For example, when tracking the growth and development of globular cell clusters and organoids, their speed and reliability will help you achieve ideal results.

Thanks to the following advantages, accurate intermittent uptake multi site experiments and cell change tracking can be achieved using THUNDER Imager:

Reliable Z-axis offset correction achieved through adaptive focus control (AFC)
Software autofocus that compensates for changes in sample position
Accurate Z-positioning (closed-loop focusing) with a repeatability of up to 20 nm
By using a new quantum platform, more data information can be obtained in a shorter amount of time. This stage has no shaking and can quickly and accurately move to all positions (e.g. 10 positions/second), with excellent reproducibility (<± 0.25 µ m).
Reliable daily data intake

THENER Imager 3D Live Cell and THENER Imager 3D Cell Culture can always keep the focus on live cells, achieving reliable image data capture.

Due to drift, morphological changes, or growth, live cell imaging is often very challenging. Drift is caused by vibration, mechanical creep, or temperature fluctuations. Drift and cellular changes can both reduce the reliability of captured image data, as focusing is a problem. But thanks to adaptive focus control (AFC), closed-loop focusing, and software autofocus capabilities, the THENER Imager 3D Live Cell and THENER Imager 3D Cell Culture can reliably maintain focus for your porous experiments.