There are many derivative detection methods for aflatoxin, most of which are pre column trifluoroacetic acid derivatives or post column iodine chemical derivatives. However, due to the cumbersome preparation of derivative reagents and complex instrument operation, these methods are increasingly being replaced by photochemical derivatives. The Huang Aflatoxin Analysis Liquid Chromatography System launched by Dalian Yilite Analytical Instrument Email Company uses photochemical derivatization method for detection. This method has the advantages of easy operation, no need for derivatization reagents, avoiding contact with du reagents, and environmental friendliness.

Recommended Configuration for EClassical 3100 High Performance Liquid Chromatography System for Aflatoxin Analysis

Serial Number	name	quantity
Equivalent system instrument configuration
1	fluorescence detector	1 unit
2	P3100 high-pressure constant current pump	1 unit
3	UV3100 UV Visible Detector (optional)	1 unit
4	Rheodyne 7725i high-pressure six way injection valve	1 piece
5	ZJ-1 valve bracket	1 piece
6	W5100 Chromatographic Data Workstation	1 set
7	TD-1-15 gradient mixer (optional)	1 piece
8	TP3100 solvent tray	1 unit
9	AD adapter	1 set
10	C18 5 µ m chromatographic column	1 unit
Instrument configuration for post column derivative system
11	Post column derivatizer for photochemistry	1 set
Configuration (more features, user-friendly configuration, higher efficiency)
12	O3100 chromatographic column constant temperature box
13	S3100 automatic sampler	1 unit
14	DG3100 online deaerator	1 unit

Recommended configuration of iChrom 5100 high-performance liquid chromatography system for aflatoxin analysisplace

Serial Number	name	quantity
Equivalent system instrument configuration
1	fluorescence detector	1 unit
2	P5102 High pressure constant current pump	1 unit
3	D5101 UV Visible Detector (optional)	1 unit
4	Rheodyne 7725i high-pressure six way injection valve	1 piece
5	VB5101 valve bracket	1 piece
6	W5100 Chromatographic Data Workstation	1 set
7	M5101 System Organizer	1 unit
8	AD module	1 set
9	C18 5 µ m chromatographic column	1 unit
10	5100 System Toolkit	1 set
Instrument configuration for post column derivative system
11	Post column derivatizer for photochemistry	1 set
Configuration (more features, user-friendly configuration, higher efficiency)
12	O5100 chromatographic column constant temperature box	1 unit
13	S5101 automatic sampler	1 unit
14	M5102 System Organizer	1 unit

Note: Other models of high-performance liquid chromatography with equivalent configuration

List of main chemical reagents and standard samples

Serial Number	reagent	purity
1	Yi nitrile	HPLC
2	Jia Chun	HPLC
3	sodium chloride	analytical pure
4	deionized water	18.2MΩ
5	Huang Aflatoxin Mixed Standard Solution	Bai Lingwei

List of main sample pre-treatment equipment

Serial Number	name	Specification and model
1	Solvent filter	1000mL
2	Diaphragm Vacuum Pump	0.08MPa,160W
3	Ultrasonic cleaner	3L/6L, 40/60KHz, 120W
4	universal high-speed smashing machines	Speed greater than 10000r/min
5	Test sieve	Second sieve
6	Precision electronic balance	Sensitivity is one ten thousandth
7	High speed homogenizer	Speed greater than 10000r/min
8	immunoaffinity column	Huang Aflatoxin Immunoaffinity Column
9	Pump flow control rack	Matching immune affinity column

performance index

analysis speed	Analysis time<30 minutes
linear range	B1、 G1The linear relationship is good within the concentration range of 0.2 μ g/L to 20.0 μ g/L, B2、 G2The linear relationship is good within the concentration range of 0.06 μ g/L to 6.0 μ g/L
detection limit	B10.03 μ g/kg, G10.08 μ g/kg、B20.01 μ g/kg, G20.03 μ g/kg